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Image Search Results
Journal: bioRxiv
Article Title: RetroCHMP3 Blocks Budding of Enveloped Viruses Without Blocking Cytokinesis
doi: 10.1101/2020.08.30.273656
Figure Lengend Snippet: (A) Schematic of putative regulatory elements upstream of retroCHMP3 ORF in Mus musculus identified by transcription factor binding site prediction tools. Numbers indicate nucleotides relative to retroCHMP3 start codon. LTR – long terminal repeat, MuRRS – murine retrovirus-related sequence, ERV – endogenous retrovirus. (B) RT-PCR detection of retroCHMP3 RNA in mouse cardiac endothelial cells (MCECs) with and without interferon stimulation. RetroCHMP3 bands were excised, cloned, and sequenced to verify a match with the retroCHMP3 sequence. Representative gel from three independent biological repeats. RT – reverse transcriptase. (C) Droplet digital PCR (ddPCR) detection of retroCHMP3 (red) and RNAse L (grey, positive control) RNA in mouse cardiac endothelial cells (MCECs) with and without interferon stimulation. The housekeeping gene succinate dehydrogenase complex, subunit A (SDHA) was used for normalization. Each line represents one independent biological repeat.
Article Snippet:
Techniques: Binding Assay, Sequencing, Reverse Transcription Polymerase Chain Reaction, Clone Assay, Reverse Transcription, Digital PCR, Positive Control